Resequencing the Escherichia coli genome by GenoCare single molecule sequencing platform

Luyang Zhao; Liwei Deng; Gailing Li; Huan Jin; Jinsen Cai; Huan Shang; Yan Li; Andrew X. Yang; Chen Fang; Zhi Yun Zhao; Guanjie Xu; Wuxing Liu; Siyu Liu; Guobing Xiang; Bin Liu; Weibin Xu; Lidong Zeng; Renli Zhang; Huan Zhao; Ping Chun Wu; Zhiliang Zhou; Jiao Zheng; Pierre Ezanno; Weiyue Chen; Qin Yan; Michael W. Deem; Jun Yu; Jiankui He

doi:10.1101/163089

GenomicsbioRxivJul 13, 2017

Preprint

Resequencing the Escherichia coli genome by GenoCare single molecule sequencing platform

Luyang Zhao iD, Liwei Deng iD, Gailing Li iD, Huan Jin, Jinsen Cai iD, Huan Shang iD, Yan Li iD, Andrew X. Yang iD, Chen Fang iD, Zhi Yun Zhao, Guanjie Xu, Wuxing Liu iD, Siyu Liu iD, Guobing Xiang, Bin Liu iD, Weibin Xu iD, Lidong Zeng iD, Renli Zhang iD, Huan Zhao iD, Ping Chun Wu iD, Zhiliang Zhou, Jiao Zheng iD, Pierre Ezanno, Weiyue Chen iD, Qin Yan, , Jiankui He iD

Abstract

Next generation sequencing (NGS) has revolutionized life sciences research. Recently, a new class of third-generation sequencing platforms has arrived to meet increasing demands in the clinic, capable of directly measuring DNA and RNA sequences at the single-molecule level without amplification. Here, we use the new GenoCare single molecule sequencing platform from Direct Genomics to resequence the E. coli genome and show comparable performance to the Illumina MiSeq system. Our platform detects single-molecule fluorescence by total internal reflection microscopy, with sequencing-by-synthesis chemistry. With a consensus sequence of 99.71% nucleotide identity to that of the Illumina MiSeq systems, GenoCare was determined to be a reliable platform for single-molecule sequencing, with strong potential for clinical applications.

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