Reproductive biology and impacts on aquatic species

This study identifies genetic and molecular mechanisms in Atlantic salmon brains associated with early maturation, linking the Hippo pathway to reproductive timing.

This study identifies paternal-effect genes in fish by comparing progeny from fresh and cryopreserved semen, finding that cryopreservation upregulates genes involved in visual development.

The paper introduces a high-throughput platform for functional studies of peptide hormones in fish, demonstrating its ability to rescue growth and fertility defects through genetic manipulation.

The paper develops methods to cryopreserve sperm from two sea urchin species, maintaining fertilization capacity and producing viable larvae after multiple freeze-thaw cycles.

The paper develops a Real-Time PCR protocol for sex identification in Atlantic salmon, demonstrating accuracy and applicability to degraded DNA samples.

The paper identifies the arrival and interactions of vascular, lymphatic, pericyte, and macrophage cells in the zebrafish bipotential gonad during early development.

This study investigates the effects of dre-miR-210-5p in zebrafish ovarian cells using an in vitro model, identifying thousands of differentially expressed genes and their involvement in reproductive and immune pathways.

This study identifies Adad1 as essential for germ cell maintenance and meiosis in zebrafish, contrasting with its role in mice.

The paper identifies bouncer as a key protein enabling immature oocyte fertilization in guppies, a unique reproductive strategy not observed in other fish species.

This study uses CRISPR/Cas9 to disrupt vtg2 in zebrafish, revealing its essential role in vitellin membrane formation and early embryonic survival.

The paper identifies specific amino acid residues and N-glycosylation patterns in Bouncer proteins that mediate species-specific sperm-egg compatibility between medaka and zebrafish.

This study shows that in medaka, photoperiod regulates gonadotrope cell division and hormone production through melatonin, TSH, and folliculostellate cells.

This study characterizes ovarian development stages and identifies VgR gene silencing as a factor reducing fecundity in parthenogenetic Artemia.

The paper validates a male-specific DNA marker (CgaY1) in African catfish, confirming an XX/XY sex determination system and demonstrating its accuracy in predicting sex.

This paper demonstrates the sterilization of Atlantic salmon embryos using antisense oligonucleotides targeting the dead end gene, resulting in germ cell-depleted fish with normal morphology but no gametes.

This study investigates how sex steroids regulate gonadotrope cell number and function in medaka, revealing sexually dimorphic mechanisms involving mitosis and transdifferentiation.

This study shows that bmp15 deficiency in zebrafish causes follicle arrest and sex reversal, which can be rescued by inhibin mutation, revealing interactions between bmp15 and the activin-inhibin system in folliculogenesis.

This study shows that loss of Gdf9 in zebrafish causes early follicle arrest and sex reversal, which can be rescued by inhibin mutation, suggesting Gdf9 acts via the activin-inhibin system.

This study shows that cd9b mutation in zebrafish reduces egg numbers and fertilization, with different effects depending on the gender of the mutant.

The paper identifies sex-specific miRNAs in zebrafish gonads that respond to heat treatment, suggesting their potential as epigenetic markers for environmental exposure.

The paper develops antibodies and an immunofluorescence method to accurately identify spermatogenic cell types in fish testis samples across multiple species.

This study identifies distinct biological processes in zebrafish gonad differentiation at 25 and 30 dpf, revealing metabolic and immune-related pathways in ovary and testis development respectively.

The paper uses single-cell RNA sequencing to analyze zebrafish ovarian cells, identifying novel cell subpopulations and gene functions involved in ovarian development.

The paper develops an antibody targeting vitellogenin in the red spiny lobster Palinurus elephas using its mRNA molecular pathway.

The paper investigates the triggers of clonal gametogenesis in hybrids, finding that it is initiated by intrinsic signals in hybrid germ cells but requires a female gonadal environment for execution.

The paper shows how duplication and sequence diversification of acrosomal proteins in abalone contribute to molecular diversity in fertilization.

This study identifies differences in proteomic profiles between good and poor quality Atlantic halibut eggs, showing impaired transcription and protein folding in poor quality eggs affecting early development.

The paper identifies sex-specific genes in sea urchin gonads that are expressed before metamorphosis, revealing early sex determination and unique gonad-specific transcripts.

This study shows that zebrafish Spaca6 is essential for fertilization by regulating Dcst2 levels and enabling sperm-egg membrane binding.

The paper identifies genes in the germline-specific region of the sea lamprey genome and shows their enrichment in testis expression and potential role in sex determination.