Time evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA
A. Gallud, M. J. Munson, K. Liu, A. Idstrom, H. M. Barriga, S. Tabaei, N. Aliakbarinodehi, M. Ojansivu, Q. Lubart, J. J. Doutch, M. N. Holme, L. Evenas, L. Lindfors, M. M. Stevens, A. Collen, , E. K. Esbjorner
This study models protein corona formation on LNPs and identifies time-dependent steps that affect their uptake and mRNA delivery, showing that pre-incubation in serum can eliminate a lag-time.
bioRxiv · Aug 20, 2021 · PreprintBiophysics