F. Hook

Time Resolved Inspection of Ionizable-Lipid Facilitated Lipid Nanoparticle Disintegration and Cargo Release at an Endosomal Membrane Mimic

This study uses TIRF imaging to examine pH-induced LNP fusion and mRNA release, showing delayed fusion onset and rapid cargo escape, with implications for optimizing mRNA delivery.

Single-molecule Dynamic In-Solution Inhibition Assay: A Method for Full Kinetic Profiling of Drug Candidate Binding to GPCRs in Native Membranes

The paper introduces a method to profile drug binding kinetics to GPCRs in native membranes using single-molecule imaging, demonstrating its application with beta2AR and drug candidates.

Time evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA

This study models protein corona formation on LNPs and identifies time-dependent steps that affect their uptake and mRNA delivery, showing that pre-incubation in serum can eliminate a lag-time.