BioengineeringbioRxivPreprint
A rapid and tunable method to temporally control Cas9 expression enables the identification of essential genes and the interrogation of functional gene interactions in vitro and in vivo.
Serif Senturk, Nitin H. Shirole, Dawid D. Nowak…·Raffaella Sordella
This paper introduces a method to temporally control Cas9 expression using a destabilizing domain, enabling precise gene editing and functional interaction studies in vitro and in vivo.