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Annette B. Iturralde

National Institutes of Health

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Approach:experimental assay· 1

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Cory A. WelleriD· 1 shared Meru J. SadhuiD· 1 shared Simone M. Giovanetti· 1 shared

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Cory A. WellerORCID

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GeneticsProceedings of the National Academy of SciencesPeer-reviewedNov 21, 2024

Comprehensive deletion scan of anti-CRISPR AcrIIA4 reveals essential and dispensable domains for Cas9 inhibition

Annette B. Iturralde, Cory A. Weller, Simone M. Giovanetti, Meru J. Sadhu

The paper develops a high-throughput method to identify essential and dispensable domains in AcrIIA4 by generating and testing precise deletions, revealing that PAM binding inhibition is key while catalytic domain interaction is dispensable.

4 citations

experimental assay

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