BiochemistrybioRxiv
Quantifying the spatiotemporal dynamics of IRES versus Cap translation with single-molecule resolution in living cells
A. L. Koch, L. Aguilera, T. Morisaki…·B. E. Munsky
The paper develops a bicistronic biosensor to quantify IRES and Cap-dependent translation dynamics in living cells, revealing distinct kinetic behaviors and stress-induced shifts in translation efficiency.
DOI 10.1038/s41594-020-0504-7