A bead-based GPCR phosphorylation immunoassay for high-throughput ligand profiling and GRK inhibitor screening
J. Kaufmann, N. K. Blum, F. Nagel, A. S. Anna Schuler, J. Drube, C. Degenhart, J. Engel, J. E. Eickhoff, P. Dasgupta, S. Fritzwanker, M. Guastadisegni, C. Schulte, E. Miess-Tanneberg, H. M. Maric, M. Spetea, , S. Schulz
This paper develops a bead-based immunoassay to quantify GPCR phosphorylation for ligand profiling and GRK inhibitor screening, demonstrating its utility with selective inhibitors.
bioRxiv · Jul 25, 2022Pharmacology & Toxicology