BioengineeringbioRxiv
Harnessing endogenous repair mechanisms for targeted gene knock-in of bovine embryos
J. R. Owen, S. L. Hennig, B. R. McNabb…·A. L. Van Eenennaam
This study compares gene knock-in efficiency in bovine zygotes using homology-directed repair (HDR) and homology-mediated end joining (HMEJ) templates, finding HMEJ achieves higher knock-in rates and lower mosaicism.
DOI 10.1038/s41598-020-72902-x