Homology mediated end joining enables efficient non-viral targeted integration of large DNA templates in primary human T cells
B. R. Webber, M. J. Johnson, N. S. Slipek, W. S. Lahr, A. P. DeFeo, J. G. Skeate, X. Qiu, B. Rathmann, M. D. Diers, B. Wick, T. Henley, M. Choudhry, R. S. McIvor, B. S. Moriarity
This paper describes a non-viral method for efficiently integrating large DNA into primary human T cells using CRISPR-Cas9 and homology-mediated end joining, achieving high functionality and anti-tumor activity.
bioRxiv · Nov 12, 2021 · PreprintBioengineering