Inducible CRISPRi-based operon silencing and selective in trans gene complementation in Borrelia burgdorferi
B. T. Murphy, J. J. Wiepen, H. He, A. S. Pramanik, J. M. Peters, B. Stevenson, W. R. Zueckert
This paper develops an inducible CRISPRi system for gene repression in Borrelia burgdorferi and demonstrates in trans complementation to study gene function.
bioRxiv · Dec 5, 2022Microbiology