Synthetic BiologyACS Synthetic BiologyPeer-reviewed
Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias profiling
Vladimir Potapov, Jennifer L. Ong, Rebecca B. Kucera…·Gregory J. S. Lohman
The paper develops a method to optimize Golden Gate assembly by profiling ligation fidelity and bias, enabling efficient assembly of up to 24 fragments in a single reaction.
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