Vladimir Potapov

The paper identifies cellular factors influencing DGR activity in E. coli through reconstitution and screens, revealing mutagenic contexts and orientation preferences that enhance editing efficiency.

The paper describes a method for efficiently constructing genes from low-cost oligonucleotide mixtures using Data-optimized Assembly Design and Golden Gate Assembly.

This study evaluates the fidelity of uridine analog incorporation during in vitro transcription using different RNA polymerases and introduces a protocol to improve incorporation fidelity without affecting RNA yield.

This study shows that combining CbAgo with RecBexo-C enables efficient in vitro cleavage of double-stranded DNA at specific sequences, generating custom single-stranded overhangs for DNA ligation.

The paper describes a method to rapidly assemble a 40 kb genome from 52 DNA parts using optimized Golden Gate assembly, resulting in infectious phage particles.

The paper develops a method to optimize Golden Gate assembly by profiling ligation fidelity and bias, enabling efficient assembly of up to 24 fragments in a single reaction.