C. Lartigue

Comparative genomics of Mycoplasma feriruminatoris, a fast-growing pathogen of wild Caprinae

This paper compares the genomes of 14 Mycoplasma feriruminatoris strains to understand its genetic features and evolutionary relationships.

Evolution of the CRISPR-Cas9 defence system in Mycoplasma gallisepticum following colonization of a novel bird host

This study tracks the evolution of CRISPR-Cas9 in Mycoplasma gallisepticum after it shifted to a novel bird host, showing changes in spacers and PAM recognition, and a loss of CRISPR-Cas function over time.

Unblocking genome editing of major animal mycoplasmas using CRISPR/Cas9 base editor systems

This paper develops a CRISPR/Cas9 base editor system to efficiently edit the genomes of three major mycoplasma pathogens, enabling the study of virulence factors and vaccine development.

Tuning spherical cells into kinking helices in wall-less bacteria

This study expresses Spiroplasma MreB isoforms and fibrils in Mycoplasma to induce helical shapes and kink-like motility, revealing MreB5's role in helicity and suggesting additional factors are needed for swimming.

CReasPy-cloning: a method for simultaneous cloning and engineering of megabase-sized genomes in yeast using the CRISPR-Cas9 system

The paper introduces CReasPy-cloning, a method that uses CRISPR-Cas9 and yeast homologous recombination to clone and engineer bacterial genomes in yeast, demonstrating efficient multi-locus editing.

Removal of a subset of non-essential genes fully attenuates a highly virulent Mycoplasma strain

This paper attenuates a highly virulent Mycoplasma strain by deleting non-essential genes and demonstrates its safety in vivo using a goat model.