C. Thiel

Digitalized organoids: integrated pipeline for 3D high-speed analysis of organoid structures using multilevel segmentation and cellular topology

This paper introduces a pipeline for 3D organoid analysis using multilevel segmentation and cellular topology to quantify morphology and topology changes under various stress conditions.

The non-muscle actinopathy-associated mutation E334Q in cytoskeletal gamma-actin perturbs interaction of actin filaments with myosin and ADF/cofilin family proteins

This study characterizes the mutation E334Q in gamma-actin, showing it affects interactions with myosin and ADF/cofilin proteins, potentially contributing to non-muscle actinopathy.

Simple, rapid, and sensitive quantification of dolichyl phosphates using phosphate methylation and reverse-phase liquid chromatography-high resolution mass spectrometry

This paper introduces a method to quantify dolichyl phosphates using phosphate methylation and RPLC-MS, applied to yeast, HeLa cells, and fibroblasts from CDG patients and controls.

Targeted Proteomics Reveals Quantitative Differences in Low Abundance Glycosyltransferases of Patients with Congenital Disorders of Glycosylation

This study develops a targeted proteomics assay to quantify low-abundance glycosyltransferases in CDG patients, revealing specific protein level changes in ALG1 and ALG2 mutants.

Glycosyltransferase POMGNT1 deficiency affects N-cadherin-mediated cell-cell adhesion

This study shows that POMGNT1 deficiency increases N-cadherin levels and alters its N-glycan structures, enhancing cell-cell adhesion and activating EMT-like signaling pathways.