Chamitha J. Weeramange

This study shows that fructose-1-kinase in E. coli can reverse its reaction to generate F-1-P from F-1,6-BP, binds Cra, and influences metabolism and biofilm formation beyond fructose catabolism.

The paper compares in vitro and in vivo activities of engineered LacI-based repressor proteins, showing that DNA looping and operator sequence variation significantly affect their function.