Author

Chardasia L. Smith

Florida State University

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Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 1 Organism:human· 1

Frequent Co-Authors

David M. GilbertiD· 1 shared Emily A. ShieliD· 1 shared Hong LiiD· 1 shared Kyle N. KleiniD· 1 shared Mitchell O. RothiD· 1 shared Travis H. HandiD· 1 shared

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2021-2021 · peak 1/yr

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1 shared paperSynthetic Biology

David M. GilbertORCID

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1 paper

Recent·Most cited

Synthetic BiologyThe CRISPR JournalPeer-reviewedApr 1, 2021

Catalytically Enhanced Cas9 through Directed Protein Evolution

Travis H. Hand, Mitchell O. Roth, Chardasia L. Smith…·Hong Li

This paper describes a directed protein evolution method to enhance the catalytic efficiency of CRISPR-Cas9 variants, showing up to 4-fold improvement in AceCas9 and 2-fold increase in HDR-based gene insertion for SpyCas9.

16 citations

humanexperimental assay

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