An intact amber-free HIV-1 system for in-virus protein bioorthogonal click labeling that delineates envelope conformational dynamics
Y. Ao, J. R. Grover, Y. Han, G. Zhong, W. Qin, D. Ghimire, M. A. Haque, R. Bhattacharjee, B. Zhang, J. Arthos, E. A. Lemke, P. D. Kwong, M. Lu
This paper develops an amber-free HIV-1 system for in-virus protein labeling, enabling smFRET analysis of Env conformational dynamics without disrupting structural integrity.
bioRxiv · Feb 28, 2023 · PreprintMicrobiology