Author

E. A. Lemke

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 5 Biochemistry· 1 Cell Biology· 1 Microbiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Research Focus:cell signaling & trafficking· 3 Assay:fluorescence / live-cell microscopy· 3 Organism:human· 3 Research Focus:protein folding & dynamics· 3 Approach:statistical model· 2 Approach:biophysical modeling· 1 Research Focus:chromatin & epigenetic regulation· 1

Frequent Co-Authors

M. Yu· 3 shared T. Scheidt· 3 shared M. Brzezinski· 2 shared A. A. Watson· 1 shared A. J. Gralak· 1 shared A. L. Chambers· 1 shared A. Obarska-Kosinska· 1 shared A. Radenovic· 1 shared

Activity by Year

2017-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 13 mutual co-author links

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3 shared papersBiophysics

M. Yu

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Author profile →

8 papers

Recent·Most cited

Chaperone quality control in liquid-phase separated organelles

T. Scheidt, E. A. Lemke

This study shows that small heat shock proteins inhibit FUS and TDP-43 nano-cluster formation and target aggregation interfaces, highlighting their role in preventing neurodegenerative protein aggregation.

bioRxiv · Jan 23, 2024 · PreprintBiophysics

Protein-specific crowding accelerates aging in phase-separated droplets

M. Brzezinski, P. G. Argudo, T. Scheidt, M. Yu, E. A. Lemke, J. Michels, S. H. Parekh

This study shows that PEG affects phase-separated droplets differently depending on the protein, accelerating Nup98 maturation but stabilizing BSA droplets.

bioRxiv · Dec 10, 2023 · PreprintBiophysics

An intact amber-free HIV-1 system for in-virus protein bioorthogonal click labeling that delineates envelope conformational dynamics

Y. Ao, J. R. Grover, Y. Han, G. Zhong, W. Qin, D. Ghimire, M. A. Haque, R. Bhattacharjee, B. Zhang, J. Arthos, E. A. Lemke, P. D. Kwong, M. Lu

This paper develops an amber-free HIV-1 system for in-virus protein labeling, enabling smFRET analysis of Env conformational dynamics without disrupting structural integrity.

bioRxiv · Feb 28, 2023 · PreprintMicrobiology

Deciphering the conformations and dynamics of FG-nucleoporins in situ

M. Yu, M. Heidari, S. Mikhaleva, P. S. Tan, S. Mingu, R. Hao, C. D. Reinkermeier, A. Obarska-Kosinska, M. Siggel, M. Beck, G. Hummer, E. A. Lemke

This study uses fluorescence microscopy and modeling to reveal the conformation and dynamics of FG-NUPs within the nuclear pore complex, showing good-solvent conditions facilitate transport.

bioRxiv · Jul 7, 2022Biophysics

Role of Solvent Compatibility in the Phase Behavior of Binary Solutions of Weakly Associating Multivalent Polymers

J. J. Michels, M. Brzezinski, T. Scheidt, E. A. Lemke, S. Parekh

The paper investigates how solvent compatibility affects phase behavior in binary solutions of multivalent polymers, showing that it modulates isothermal liquid-liquid phase separation.

bioRxiv · Sep 9, 2021Biophysics

Bioorthogonal red and far-red fluorogenic probes for wash-free live-cell and super-resolution microscopy

P. Werther, K. Yserentant, F. Braun, K. S. Grussmayer, V. Navikas, M. Yu, Z. Zhang, M. J. Ziegler, C. Mayer, A. J. Gralak, M. Busch, W. Chi, F. Rominger, A. Radenovic, X. Liu, , R. Wombacher

The paper develops new fluorogenic probes for live-cell and super-resolution microscopy with minimal label size and efficient quenching.

bioRxiv · Aug 7, 2020Cell Biology

Molecular determinants of large cargo transport into the nucleus

E. A. Lemke, G. Paci

The paper investigates the molecular mechanisms of nuclear import for large cargoes, showing that transport efficiency scales non-linearly with size and requires a minimum of 10 NLSs.

bioRxiv · Jul 6, 2019Biophysics

Architecture of TAF11/TAF13/TBP complex suggests novel regulatory state in General Transcription Factor TFIID function

K. Gupta, A. A. Watson, T. Baptista, E. Scheer, A. L. Chambers, C. Koehler, J. Zou, I. Obong-Ebong, E. Kandiah, A. Temblador, A. Round, E. Forest, P. Man, C. Bieniossek, E. D. Laue, , I. Berger

The paper identifies a ternary TAF11/TAF13/TBP complex and reveals a novel regulatory state in TFIID function through structural and biochemical analyses.

bioRxiv · Jul 26, 2017Biochemistry