BiochemistrybioRxiv
Homology Directed Repair by Cas9:Donor Co-localization in Mammalian Cells
P. J. Roche, H. Gytz, F. Hussain…·U. D. Akavia
The paper improves HDR efficiency in mammalian cells using Cas9 fused to a monoavidin domain and PEI delivery, achieving up to 90% HDR in three loci.
DOI 10.1089/crispr.2018.0045