GenomicsbioRxivPreprint
Mismatch tolerance of a gRNA for CRISPR-based gene activation confers broad activity critical for cell reprogramming
Samuel J. Reisman, Wei Zhu, Samantha E Miller…·Charles A. Gersbach
The paper identifies a CRISPRa gRNA with broad off-target activity that enables genome-wide transcriptional reprogramming, showing that mismatch tolerance and PAM-proximal bases determine binding specificity.
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