Synthetic BiologybioRxivPreprint
Predictive genome-wide CRISPR-mediated gene downregulation for enhanced bioproduction
Ian Sofian Yunus, David N. Carruthers, Yan Chen…·Taek Soon Lee
This study introduces two methods, FluxRETAP and VAMMPIRE, to improve CRISPRi-mediated gene downregulation for bioproduction by enhancing target identification and multiplexed construct assembly.