Changes in Active Site Loop Conformation Relate to the Transition toward a Novel Enzymatic Activity
P. Jacquet, R. Billot, A. Shimon, N. Hoekstra, C. Bergonzi, A. Jenks, E. Chabriere, D. Daude, M. Elias
The paper redesigns the active site of lactonase SsoPox to enhance phosphotriester catalytic activity, revealing that loop conformation changes are critical for activity shifts.
bioRxiv · May 22, 2023Biochemistry