BiochemistrybioRxiv
Identification of Transient Intermediates During Spliceosome Activation by Single Molecule Fluorescence Microscopy
X. Fu, H. Kaur, M. L. Rodgers…·A. Hoskins
This study uses single molecule fluorescence to identify a transient intermediate in yeast spliceosome activation involving Lsm2-8 and the NTC, revealing kinetic interactions not seen in cryo-EM structures.
DOI 10.1073/pnas.2206815119