Establishment of a CPER Reverse Genetics System for Powassan Virus Defines Attenuating NS1 Glycosylation Sites and an Infectious NS1-GFP11 Reporter Virus
J. N. Conde, G. E. Himmler, M. C. Mladinich, Y. X. Setoh, A. A. Amarilla, W. R. Schutt, N. Saladino, E. E. Gorbunova, D. J. Salamango, E. Wimmer, H. K. Kim, E. R. Mackow
This paper establishes a reverse genetics system for Powassan virus, identifies attenuating NS1 glycosylation sites, and creates an infectious NS1-GFP11 reporter virus to study viral functions.
bioRxiv · May 3, 2023Microbiology