Synthetic BiologyACS Synthetic BiologyPeer-reviewed
PHYCUT: Scalable multiplex CRISPR/Cas9 editing forgenome engineering in the diatom Phaeodactylumtricornutum
Emily E. Stuckless, Gai Li, Samuel S. Slattery…·David R. Edgell
The paper develops PHYCUT, a CRISPR/Cas9 system for multiplex genome editing in the diatom Phaeodactylum tricornutum, demonstrating its use to reduce fucosylation of secreted proteins.
DOI 10.1021/acssynbio.5c00843