Author

G. Vicidomini

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 8 Bioengineering· 4

Signature Assays, Organisms & Methods

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Assay:fluorescence / live-cell microscopy· 8 Research Focus:imputation· 7 Approach:microscopy & imaging analysis· 6 Approach:experimental assay· 5 Assay:imaging· 5 Research Focus:dimensionality reduction· 3 Approach:deep learning· 2 Organism:human· 2

Frequent Co-Authors

G. Tortarolo· 9 shared E. Slenders· 6 shared A. Diaspro· 5 shared M. Castello· 5 shared E. Perego· 4 shared P. Bianchini· 4 shared A. Tosi· 3 shared C. J. R. Sheppard· 3 shared

Activity by Year

2018-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 41 mutual co-author links

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9 shared papersBiophysics

G. Tortarolo

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Author profile →

12 papers

Recent·Most cited

4D Single-Particle Tracking with Asynchronous Read-Out SPAD-Array Detector

A. Bucci, G. Tortarolo, M. O. Held, L. Bega, E. Perego, F. Castagnetti, I. Bozzoni, E. Slenders, G. Vicidomini

This paper introduces a real-time 3D single-particle tracking technique combining localization and fluorescence lifetime measurement using a SPAD array detector, achieving 30 nm precision and microsecond resolution.

bioRxiv · Aug 25, 2023 · PreprintBiophysics

A Compact and Effective Photon-Resolved Image Scanning Microscope

G. Tortarolo, A. Zunino, S. Piazza, M. Donato, S. Zappone, A. Pierzynska-Mach, M. Castello, G. Vicidomini

This paper introduces a data-acquisition module for fluorescence LSM that enhances single-photon imaging capabilities, enabling super-resolution and multi-species imaging.

bioRxiv · Jul 28, 2023Biophysics

Content-enriched fluorescence lifetime fluctuation spectroscopy to study bio-molecular condensate formation

E. Perego, S. Zappone, F. Castagnetti, D. Mariani, E. Vitiello, J. Rupert, E. Zacco, G. G. Tartaglia, I. Bozzoni, E. Slenders, G. Vicidomini

This paper introduces a fluorescence lifetime fluctuation spectroscopy framework to study bio-molecular condensate formation in live cells under oxidative stress.

bioRxiv · Jun 9, 2023 · PreprintBiophysics

Focus-ISM for Sharp and Gentle Super-Resolved Microscopy

G. Tortarolo, A. Zunino, F. Fersini, M. Castello, S. Piazza, C. J. R. Sheppard, P. Bianchini, A. Diaspro, S. Koho, G. Vicidomini

The paper introduces focus-ISM, a method combining STED and ISM to improve super-resolution microscopy for live and thick samples without compromising image quality.

bioRxiv · Apr 28, 2022Biophysics

ISM-FLUX: MINFLUX with an array detector

E. Slenders, G. Vicidomini

ISM-FLUX improves MINFLUX by combining structured illumination and detection, achieving sub-15 nm localization over 800 nm FOV with 100 photons.

bioRxiv · Apr 19, 2022 · PreprintBiophysics

The BrightEyes-TTM: an Open-Source Time-Tagging Module for Single-Photon Microscopy

A. Rossetta, E. Slenders, M. Donato, E. Perego, F. Diotalevi, L. Lanzano', S. V. Koho, G. Tortarolo, M. Crepaldi, G. Vicidomini

The paper introduces an open-source time-tagging module for single-photon microscopy, enabling high-precision temporal tagging and supporting new imaging techniques.

bioRxiv · Oct 11, 2021Bioengineering

EVALUATION OF STED SUPER-RESOLUTION IMAGE QUALITY BY IMAGE CORRELATION SPECTROSCOPY (QuICS)

E. Cerutti, M. D'Amico, I. Cainero, G. I. Dellino, M. Faretta, G. Vicidomini, P. G. Pelicci, P. Bianchini, A. Diaspro, L. Lanzano'

The paper introduces an ICS-based algorithm to evaluate STED image quality, finding that increasing STED power improves resolution but reduces contrast, while SPLIT methods enhance resolution and contrast.

bioRxiv · Aug 30, 2021Biophysics

Cooled SPAD array detector for low light-dose fluorescence laser scanning microscopy

E. Slenders, E. Perego, M. Buttafava, G. Tortarolo, E. Conca, S. Zappone, A. Pierzynska-Mach, F. Villa, E. M. Petrini, A. Barberis, A. Tosi, G. Vicidomini

This paper introduces a cooled SPAD array detector that reduces dark-noise, improving signal-to-noise ratio and enabling lower laser power for live-cell imaging and artifact-free fluorescence fluctuation spectroscopy.

bioRxiv · Aug 3, 2021Biophysics

Synergic Combination of Stimulated Emission Depletion Microscopy with Image Scanning Microscopy to Reduce Light Dosage

G. Tortarolo, M. Castello, S. V. Koho, G. Vicidomini

This paper combines STED microscopy with image scanning microscopy to reduce light dosage while maintaining resolution.

bioRxiv · Aug 21, 2019 · PreprintBioengineering

Easy Two-Photon Image Scanning Microscopy with SPAD Array and Blind Image Reconstruction

S. V. Koho, E. Slenders, G. Tortarolo, M. Castello, M. Buttafava, F. Villa, E. Tcarenkova, M. Ameloot, P. Bianchini, C. J. R. Sheppard, A. Diaspro, A. Tosi, G. Vicidomini

This paper introduces a two-photon image scanning microscopy (2PE-ISM) system that improves optical resolution and image quality without user calibration.

bioRxiv · Apr 27, 2019Bioengineering

Photon-separation to enhance the spatial resolution in pulsed STED microscopy

G. Tortarolo, Y. Sun, K.-W. Teng, Y. Ishitsuka, L. Lanzano, P. R. Selvin, B. Barbieri, A. Diaspro, G. Vicidomini

This paper introduces SPLIT-pSTED, a method that enhances spatial resolution in pulsed STED microscopy by separating photons without increasing STED beam intensity.

bioRxiv · Sep 4, 2018Biophysics

Image Scanning Microscopy with Single-Photon Detector Array

M. Castello, G. Tortarolo, M. Buttafava, T. Deguchi, F. Villa, S. Koho, P. Bianchini, C. J. R. Sheppard, A. Diaspro, A. Tosi, G. Vicidomini

This paper introduces an improved image scanning microscopy technique using a single-photon detector array, enabling better resolution while maintaining compatibility with existing confocal microscopes and fluorescence spectroscopy methods.

bioRxiv · Jun 2, 2018Bioengineering