P. Bianchini

Nanoscale engagement and clusterization of Programmed death ligand 1 (PD-L1) in the membrane lipid rafts of Non-Small Cell Lung Cancer cells

This study uses super-resolution microscopy to show PD-L1 clusters in lipid rafts of NSCLC cells, suggesting new immuno-oncology targets.

Focus-ISM for Sharp and Gentle Super-Resolved Microscopy

The paper introduces focus-ISM, a method combining STED and ISM to improve super-resolution microscopy for live and thick samples without compromising image quality.

EVALUATION OF STED SUPER-RESOLUTION IMAGE QUALITY BY IMAGE CORRELATION SPECTROSCOPY (QuICS)

The paper introduces an ICS-based algorithm to evaluate STED image quality, finding that increasing STED power improves resolution but reduces contrast, while SPLIT methods enhance resolution and contrast.

A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in Vero E6 cells

The paper uses multi-scale fluorescence microscopy to study SARS-CoV-2 variant entry into Vero E6 cells, finding B.1.1.7 enters faster than its ancestor due to the N501Y mutation.

Nanoscale distribution of nuclear sites analyzed by superresolution STED-ICCS

This paper introduces STED-ICCS, a method combining superresolution microscopy with image cross-correlation spectroscopy to quantify nanoscale spatial distribution of functional nuclear sites in MCF10A cells.

Volumetric Lissajous Confocal Microscopy

This paper introduces volumetric Lissajous confocal microscopy to achieve high-speed 3D imaging with tunable sampling rates and sub-millisecond acquisition times.

Easy Two-Photon Image Scanning Microscopy with SPAD Array and Blind Image Reconstruction

This paper introduces a two-photon image scanning microscopy (2PE-ISM) system that improves optical resolution and image quality without user calibration.

Enigma at the nanoscale: can the NPC act as an intrinsic reporter for isotropic expansion microscopy?

This study evaluates expansion microscopy's isotropy by analyzing nuclear pore complexes, finding uniform accuracy within 20 nm for structural studies.

Image Scanning Microscopy with Single-Photon Detector Array

This paper introduces an improved image scanning microscopy technique using a single-photon detector array, enabling better resolution while maintaining compatibility with existing confocal microscopes and fluorescence spectroscopy methods.