Cryoprotectants-assisted plunge freezing of thick brain tissue specimens for targeted physiologically relevant cryo-imaging in situ
Ash Weier, Fei Gao, Elliot T Morgan, Pingting Liu, Zephan Melville, Louis Perez, Garry Morgan, Qian Shi, Fabio A Vigil, Edward T. Eng, Alex de Marco, Lydia‐Marie Joubert, Andreas Hoenger, Michael H. B. Stowell, Oleg V. Klykov
This paper develops a cryoprotectant-assisted plunge freezing method for preserving thick mouse brain tissue for cryo-imaging, enabling high-resolution visualization of cellular structures and validating their physiological relevance.
Cell Reports Methods · Sep 1, 2026Peer-reviewedBiophysics