Alex de Marco

The paper shows that Eg5 organizes antiparallel microtubule bundles in human metaphase spindles through density-driven steric interactions, independent of spindle bipolarity.

This paper uses Bayesian ensemble reweighting on unclassified cryo-EM images to quantify RNA folding equilibria, showing agreement with bulk solution measurements.

This paper develops a cryoprotectant-assisted plunge freezing method for preserving thick mouse brain tissue for cryo-imaging, enabling high-resolution visualization of cellular structures and validating their physiological relevance.

This paper introduces a pipeline for high-resolution cryo-ET imaging using square electron beams, demonstrating improved resolution and field-of-view by reducing radiation damage and overlap.

This paper introduces a method to reduce air-water interface artifacts in cryo-EM by encapsulating proteins in hydrophilic shells, demonstrating its effectiveness with three protein examples.

This paper describes a method to simultaneously profile the transcriptome and proteome from single cells, showing moderate correlation between the two modalities.

The paper uses multimodal imaging to study the dynamic structure of the exocyst complex during exocytosis, revealing its conformational changes and tethering mechanism.

This paper introduces CryoCycle, a method for blotting, vitrifying, and reusing clipped cryoEM grids to reduce costs and improve access to cryoEM.

This paper introduces a square electron beam for TEM to improve tiling and reduce exposure unevenness in cryo-EM imaging.

This paper introduces oxygen plasma FIB-SEM as a faster and more versatile method for cellular tomography compared to gallium FIB-SEM.