BiochemistrybioRxiv
ABPP-HT* - Deep meets fast for activity-based profiling of deubiquitylating enzymes using advanced DIA mass spectrometry methods
H. B. L. Jones, R. Heilig, S. Davis…·A. Pinto-Fernandez
The paper introduces ABPP-HT*, an improved ABPP method combining high depth and throughput for profiling deubiquitylating enzymes using DIA MS, achieving consistent profiling of 35-40 DUBs with reduced missing values.
DOI 10.3390/ijms23063263