Plant BiologyPlant PhysiologyPeer-reviewed
Native ribonucleases process sgRNA transcripts to create catalytic Cas9/sgRNA complexes in planta
The paper shows that native ribonucleases process sgRNA transcripts in plants to create catalytically active Cas9/sgRNA complexes, challenging the current CRISPR/Cas9 dogma about 5' overhangs.
DOI 10.1104/pp.20.00150