H. Ebata

Activity-dependent glassy cell mechanics : Mechanical properties measured with active microrheology

This study measures mechanical properties of living cells using active microrheology and finds that cell mechanics are governed by glassy cytoplasm, with ATP depletion leading to solidification and metabolic activity promoting fluid-like behavior.

Accumulation of TERT in Mitochondria Shows Two Opposing Effects on Apoptosis

This study shows that mitochondrial TERT has opposing effects on apoptosis: it initiates cell death early but delays it later, resolving a controversy about its role.

Nuclear activation in dual-durotaxing cells on a matrix with cell-scale stiffness-heterogeneity

This study shows that mesenchymal stem cells undergoing dual-durotaxis on stiff micro-patterned gels experience amplified intracellular stress dynamics and chromatin fluctuations, which enhance cellular vigor and maintain stemness.

Avoiding tensional equilibrium in cells migrating on a matrix with cell-scale stiffness-heterogeneity

The paper investigates intracellular stress dynamics in cells migrating on stiffness-heterogeneous matrices, finding enhanced stress fluctuations on triangular patterns due to stiffness-driven migration and traction force dynamics.

Implementation of single molecule FRET for visualizing intramolecular movement in CRISPR-Cas9

This paper describes methods to visualize flexible inter-domain movements of Cas9 using single molecule FRET and discusses necessary complementary experiments for accurate analysis.