H. Nishimasu

A single CRISPR base editor to induce simultaneous C-to-T and A-to-G mutations

This paper introduces Target-ACE, a CRISPR base editor that induces both C-to-T and A-to-G mutations, showing higher efficiency than using separate editors.

Real-time observation of flexible domain movements in Cas9

This study uses single-molecule FRET to observe dynamic domain movements in Cas9, showing flexibility enables DNA cleavage and sgRNA assembly.