BiochemistryProtein SciencePeer-reviewed
Mechanism-guided mutagenesis of Rft1 to test its role as a dolichol-linked oligosaccharide scramblase in cells
George N. Chiduza, Ken‐taro Sakata, Hannah G. Wolfe…·Anant K. Menon
The paper uses computational models and mutagenesis to test if Rft1 is essential for M5-DLO scrambling in yeast, finding that scrambling is dispensable while M5-DLO binding is critical.
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