Cell BiologybioRxivPreprint
Nanobodies equipped with HaloTag variants enable rapid and straightforward one-step immunofluorescence lifetime multiplexing
László Albert, Samrat Basak, Henrike Koerner…·Felipe Opazo
The paper introduces nanobody-HaloTag constructs for multiplexed immunofluorescence imaging, enabling up to eight targets with lifetime and spectral encoding in a single step.
DOI 10.64898/2026.03.02.709025