J. Corver

Proteolytic activity of surface exposed HtrA determines its expression level and is needed to survive acidic conditions in Clostridioides difficile

This study shows that HtrA proteolytic activity affects its expression level and is necessary for C. difficile survival under acidic conditions.

In-depth specificity profiling of Pro-Pro endopeptidases (PPEPs) using combinatorial synthetic peptide libraries

The paper develops a method combining synthetic peptide libraries and mass spectrometry to profile the specificity of Pro-Pro endopeptidases (PPEPs) and identifies new substrates and specificity patterns.

Redefining the Clostridioides difficile σB regulon: σB activates genes involved in detoxifying radicals that can result from the exposure to antimicrobials and hydrogen peroxide

This study identifies genes directly regulated by σB in C. difficile and shows that antimicrobials and hydrogen peroxide induce these genes via σB-dependent mechanisms.

The C-terminal domain of Clostridioides difficile TcdC is exposed on the bacterial cell surface

This study shows the C-terminal domain of TcdC in C. difficile is extracellular, challenging its proposed role as an anti-sigma factor.

Plasmid-mediated metronidazole resistance in Clostridioides difficile

This paper identifies a plasmid, pCD-METRO, as the first reported cause of plasmid-mediated metronidazole resistance in Clostridioides difficile, showing its global dissemination and horizontal transfer potential.

Proteomic identification of Axc, a novel beta-lactamase with carbapenemase activity in a meropenem-resistant clinical isolate of Achromobacterxylosoxidans

The paper identifies Axc, a novel beta-lactamase with carbapenemase activity, in a meropenem-resistant Achromobacter xylosoxidans isolate using proteomics and confirms its role in resistance.