D. Jahn

Bacterial membrane vesicles of Pseudomonas aeruginosa activate AMPK signaling through inhibition of mitochondrial complex III

This study shows that Pseudomonas aeruginosa membrane vesicles inhibit mitochondrial complex III, activate AMPK signaling, and reduce global protein synthesis in human lung cells.

Isolation and quantification of bacterial membrane vesicles for quantitative metabolic studies using mammalian cell cultures

This study introduces a method to quantify bacterial membrane vesicles using FM4-64 dye and linear regression, showing its superiority over protein-based methods and identifying a key regulator in vesicle-mediated pathogenesis.

Increased beta2-adrenergic signaling is a targetable stimulus essential for bone healing by promoting callus neovascularization

This study identifies beta2-adrenergic signaling as a key driver of bone healing by enhancing callus neovascularization, with implications for therapeutic strategies and the risks of beta-blockers.

Clostridium difficile Toxin B activates the NLRP3 inflammasome in human macrophages, demonstrating a novel regulatory mechanism for the Pyrin inflammasome

The paper shows that C. difficile toxin B activates the NLRP3 inflammasome in human macrophages, not the Pyrin inflammasome, and that Pyrin activation can be restored with prolonged priming.

Redefining the Clostridioides difficile σB regulon: σB activates genes involved in detoxifying radicals that can result from the exposure to antimicrobials and hydrogen peroxide

This study identifies genes directly regulated by σB in C. difficile and shows that antimicrobials and hydrogen peroxide induce these genes via σB-dependent mechanisms.

Work horse strain Clostridioides difficile 630Δerm is oblivious to its anaerobic lifestyle

The study shows that the C. difficile strain 630Δerm has a mutation in PerR that causes oxidative stress response under anaerobic conditions, challenging its use as a model strain.