Author

J. Kliche

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 6 Biophysics· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Assay:proteomics· 6 Organism:human· 5 Research Focus:cell signaling & trafficking· 2 Research Focus:cell type annotation· 2 Research Focus:host-pathogen & immune response· 2 Research Focus:protein folding & dynamics· 2 Research Focus:cell-cell communication· 1

Frequent Co-Authors

Y. Ivarsson· 6 shared L. Simonetti· 5 shared P. Jemth· 4 shared F. Mihalic· 3 shared J. Nilsson· 3 shared M. Ali· 3 shared N. E. Davey· 3 shared A. Sayadi· 2 shared

Activity by Year

2020-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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6 shared papersBiochemistry

Y. Ivarsson

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Author profile →

7 papers

Recent·Most cited

Proteome-scale characterisation of motif-based interactome rewiring by disease mutations

J. Kliche, L. Simonetti, I. Krystkowiak, H. Kuss, M. Diallo, E. Rask, J. Nilsson, N. Davey, Y. Ivarsson

The paper characterizes how disease mutations in intrinsically disordered regions modulate protein interactions mediated by short linear motifs, identifying both loss and gain of interactions.

bioRxiv · Sep 18, 2023Biochemistry

Evaluation of affinity-purification coupled to mass spectrometry approaches for capture of short linear motif-based interactions

E. Kassa, S. Jamshidi, F. Mihalic, L. Simonetti, J. Kliche, P. Jemth, S. B. Lind, Y. Ivarsson

The paper evaluates biotinylated peptide pulldown and PRISMA coupled to mass spectrometry for capturing SLiM-based interactions, finding biotin-peptide pulldown more effective for validation.

bioRxiv · Oct 19, 2022Biochemistry

Large-scale identification of phospho-modulated motif-based protein-protein interactions

J. Kliche, D. H. Garvanska, L. Simonetti, D. Badgujar, D. Dobritzsch, J. Nilsson, N. E. Davey, Y. Ivarsson

The paper identifies 252 phospho-modulated short linear motif-based protein-protein interactions using a proteomic peptide-phage display library and validates several through affinity measurements and structural analysis.

bioRxiv · Jun 8, 2022 · PreprintBiochemistry

Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities

T. Kruse, C. Benz, D. H. Garvanska, R. Lindqvist, F. Mihalic, F. Coscia, R. Inturi, A. Sayadi, L. Simonetti, E. Nilsson, M. Ali, J. Kliche, A. Moliner Morro, A. Mund, E. Andersson, , Y. Ivarsson

The paper identifies coronavirus-host protein interaction motifs, revealing SARS-CoV-2 specific mechanisms and a peptide-based inhibitor that blocks infection.

bioRxiv · Apr 19, 2021Biochemistry

Proteome-scale amino-acid resolution footprinting of protein-binding sites in the intrinsically disordered regions of the human proteome

C. Benz, M. Ali, I. Krystkowiak, L. Simonetti, A. Sayadi, F. Mihalic, J. Kliche, E. Andersson, P. Jemth, N. E. Davey, Y. Ivarsson

The paper introduces HD2, a proteome-scale method for identifying and characterizing SLiM-mediated protein interactions with amino acid resolution, and provides tools for analyzing the results.

bioRxiv · Apr 13, 2021Biochemistry

Cytoplasmic short linear motifs in ACE2 and integrin beta3 link SARS-CoV-2 host cell receptors to endocytosis and autophagy

J. Kliche, M. Ali, Y. Ivarsson

Affinity measurements confirmed binding of short linear motifs in the cytoplasmic tails of ACE2 and integrin β3, linking these receptors to endocytosis and autophagy.

bioRxiv · Oct 6, 2020Biochemistry

Supertertiary protein structure affects an allosteric network

L. Laursen, J. Kliche, S. Gianni, P. Jemth

The paper investigates how supertertiary structure influences allosteric networks in PDZ domains, showing that allosteric behavior depends on the supramodular context.

bioRxiv · Mar 24, 2020Biophysics