P. Jemth

Evaluation of affinity-purification coupled to mass spectrometry approaches for capture of short linear motif-based interactions

The paper evaluates biotinylated peptide pulldown and PRISMA coupled to mass spectrometry for capturing SLiM-based interactions, finding biotin-peptide pulldown more effective for validation.

A syntenin inhibitor blocks endosomal entry of SARS-CoV-2 and a panel of RNA viruses

A syntenin inhibitor blocks endosomal entry of SARS-CoV-2 and multiple RNA viruses by preventing their uptake through receptor-mediated endocytosis.

Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs

The paper uses phage-based screening to identify 1,712 virus-host interactions mimicking host short linear motifs, revealing therapeutic targets for broad-spectrum antivirals.

Rescue of Escherichia coli auxotrophy by de novo small proteins

This study shows that de novo small proteins can rescue Escherichia coli auxotrophy by binding to RNA regulatory regions and increasing gene expression.

The dynamic properties of a nuclear coactivator binding domain are evolutionarily conserved

The paper compares the dynamic properties of an ancient NCBD from a bilaterian ancestor to human NCBD, finding conserved dynamics despite increased thermodynamic stability.

Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities

The paper identifies coronavirus-host protein interaction motifs, revealing SARS-CoV-2 specific mechanisms and a peptide-based inhibitor that blocks infection.

Proteome-scale amino-acid resolution footprinting of protein-binding sites in the intrinsically disordered regions of the human proteome

The paper introduces HD2, a proteome-scale method for identifying and characterizing SLiM-mediated protein interactions with amino acid resolution, and provides tools for analyzing the results.

CRISPR/Cas9-based inactivation of human papillomavirus oncogenes E6 and E7 induces senescence in cervical cancer cells

This study shows that CRISPR/Cas9 inactivation of HPV18 E6 and E7 in HeLa cells induces senescence, characterized by specific markers and altered p53/p21 and pRb/p21 levels.

Evolution of an interaction between disordered proteins resulted in increased heterogeneity of the binding transition state

The paper compares the binding transition states of ancestral and human IDP domains, finding increased heterogeneity in the human complex.

Supertertiary protein structure affects an allosteric network

The paper investigates how supertertiary structure influences allosteric networks in PDZ domains, showing that allosteric behavior depends on the supramodular context.

Helix formation during the coupled binding and folding of intrinsically disordered proteins monitored by synchrotron-radiation circular dichroism spectroscopy

The paper uses synchrotron-radiation circular dichroism to study helix formation during the binding of intrinsically disordered proteins, revealing kinetic phases and structural intermediates.