Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content
G. Garcia-Gimenez, A. Barakate, P. Smith, J. Stephens, S. F. Khor, M. S. Doblin, P. Hao, A. Bacic, G. B. Fincher, R. A. Burton, R. Waugh, M. R. Tucker, K. Houston
This study uses CRISPR/Cas9 to mutate barley Csl genes, revealing that HvCslF6 is essential for (1,3;1,4)-β-glucan synthesis while other family members affect cell wall composition and grain morphology.
bioRxiv · May 13, 2020Plant Biology