The paper develops a dual-species proteomics approach to study host-pathogen protein interactions and demonstrates targeted intervention using a novel inhibitor.

bioRxiv · Jul 2, 2023Biochemistry

TWISTED DWARF1 mediates myosin XI-associated vesicle trafficking required for auxin transport

J. Liu, J. Zhu, M. di Donato, P. Hao, H. Ren, M. M. Geisler

This study identifies TWD1 as an ER-actin adapter that links myosin XI-K to auxin transport, explaining defects in ABCB biogenesis and plant development.

bioRxiv · Jul 18, 2022 · PreprintCell Biology

High-density and scalable protein arrays for single-molecule proteomic studies

T. Aksel, H. Qian, P. Hao, P. F. Indermuhle, C. Inman, S. Paul, K. Chen, R. Seghers, J. K. Robinson, M. De Garate, B. Nortman, J. Tan, S. Hendricks, S. Sankar, P. Mallick

This paper develops a scalable method to create dense single-molecule protein arrays using DNA origami and surface functionalization, enabling high-sensitivity proteomic analysis.

bioRxiv · May 2, 2022 · PreprintBioengineering

THEMIS is a priming substrate of non-receptor tyrosine phosphatase PTPN6/SHP1 and plays dual roles during T cell development

J. Zhang, E. Zuo, M. Song, L. Chen, Z. Jiang, S. Chen, X. Xiong, Y. Wang, P. Hao, T. Horng, M. Zhuang, L. Zhang, H. Wang, G. Fan

This study identifies THEMIS as a substrate of SHP1 and shows it has phosphorylation-dependent and -independent roles in T cell development.

bioRxiv · Dec 21, 2021 · PreprintCell Biology

TIN2 facilitates TRF1-mediated trans- and cis-interactions on physiologically relevant long telomeric DNA

H. Pan, P. Kaur, M. Liu, P. Xu, C. Mahn, R. Barnes, Q. Tang, P. Hao, D. Bhattaram, C. You, J. Piehler, K. Weninger, R. Riehn, S. Smith, P. L. Opersko, H. Wang

This study shows TIN2 promotes TRF1-mediated DNA-DNA bridging on telomeres, stabilizing interactions that depend on telomere length and sequence.

bioRxiv · Sep 12, 2020 · PreprintBiophysics

ABCB-mediated auxin transport in outer root tissues regulates lateral root spacing in Arabidopsis

J. CHEN, Y. Hu, P. Hao, Y. Zhang, O. Roth, M. Njo, L. Sterck, Y. Hu, Y. Zhao, M. Geisler, E. Shani, T. Beeckman, S. Vanneste

The paper identifies five ABCB transporters that regulate lateral root spacing in Arabidopsis by modulating auxin oscillation amplitude in outer root tissues.

bioRxiv · Jul 22, 2020 · PreprintPlant Biology

Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content

G. Garcia-Gimenez, A. Barakate, P. Smith, J. Stephens, S. F. Khor, M. S. Doblin, P. Hao, A. Bacic, G. B. Fincher, R. A. Burton, R. Waugh, M. R. Tucker, K. Houston

This study uses CRISPR/Cas9 to mutate barley Csl genes, revealing that HvCslF6 is essential for (1,3;1,4)-β-glucan synthesis while other family members affect cell wall composition and grain morphology.

bioRxiv · May 13, 2020Plant Biology

A conserved D/E-P motif in the nucleotide binding domain of plant ABCB/PGP-type ABC transporters defines their auxin transport capacity

P. Hao, J. Xia, J. Liu, M. di Donato, K. Pakula, A. Bailly, M. Jasinski, M. Geisler

The paper identifies a conserved D/E-P motif in plant ABCB transporters that defines their auxin transport capacity and shows its role in interaction with TWD1.

bioRxiv · May 8, 2020 · PreprintBiochemistry