Author

J. Tycko

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 2 Cell Biology· 1

Signature Assays, Organisms & Methods

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Organism:human· 3 Approach:deep learning· 2 Research Focus:gene regulatory network inference· 2 Assay:whole-genome sequencing· 2 Assay:CRISPR screen· 1 Research Focus:differential expression· 1 Assay:DNA methylation· 1 Approach:genetic perturbation / CRISPR· 1

Frequent Co-Authors

M. C. Bassik· 2 shared P. D. Hsu· 2 shared A. E. Friedland· 1 shared A. Fanton· 1 shared A. S. Bhatt· 1 shared C. J. Wilson· 1 shared J. K. Pritchard· 1 shared J. Mohammed· 1 shared

Activity by Year

2018-2021 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 19 mutual co-author links

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2 shared papersBioengineering

M. C. Bassik

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Author profile →

3 papers

Recent·Most cited

Large-scale discovery of recombinases for integrating DNA into the human genome

M. G. Durrant, A. Fanton, J. Tycko, M. Hinks, S. S. Chandrasekaran, N. T. Perry, J. Schaepe, P. P. Du, P. Lotfy, M. C. Bassik, L. Bintu, A. S. Bhatt, P. D. Hsu

This paper identifies and validates new large serine recombinases (LSRs) for efficient DNA integration into human genomes, achieving higher efficiency than existing tools.

bioRxiv · Nov 5, 2021Bioengineering

A genome-wide genetic screen uncovers novel determinants of human pigmentation

V. K. Bajpai, T. Swigut, J. Mohammed, J. Tycko, S. Naqvi, M. Arreola, T. Kim, N. Arora, J. K. Pritchard, M. C. Bassik, J. Wyscoka

A genome-wide CRISPR-Cas9 screen identifies novel genes regulating melanogenesis, many upregulated in dark skin melanocytes and associated with skin color variation.

bioRxiv · Sep 29, 2021 · PreprintCell Biology

Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

J. Tycko, L. A. Barrera, N. Huston, A. E. Friedland, X. Wu, J. S. Gootenberg, O. O. Abudayyeh, V. E. Myer, C. J. Wilson, P. D. Hsu

The paper screens SaCas9 specificity using a barcoded pairwise library of sgRNAs and identifies optimal spacer lengths for off-target activity.

bioRxiv · Feb 22, 2018Bioengineering