BioengineeringbioRxiv
Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
J. Tycko, L. A. Barrera, N. Huston…·P. D. Hsu
The paper screens SaCas9 specificity using a barcoded pairwise library of sgRNAs and identifies optimal spacer lengths for off-target activity.
DOI 10.1038/s41467-018-05391-2