Cell BiologybioRxiv
Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells
R. Tsuneishi, N. Saku, S. Miyata…·A. Umezawa
This study shows that ammonia treatment enriches hepatocyte-like cells derived from human pluripotent stem cells, which can be propagated long-term in vitro while maintaining liver-specific gene expression.