GenomicsbioRxiv
Mapping protein function with CRISPR/Cas9-mediated mutagenesis
Katherine F Donovan, Mudra Hegde, Meagan Sullender…·John G Doench
The paper uses CRISPR/Cas9 mutagenesis to generate allelic variants of MEK1 and BRAF, identifying gain-of-function alleles in the MAPK signaling pathway.
DOI 10.1371/journal.pone.0170445