Author

John Tam

Hospital for Sick Children

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 3

Signature Assays, Organisms & Methods

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Research Focus:drug response / drug discovery· 2 Approach:experimental assay· 2 Research Focus:host-pathogen & immune response· 2 Organism:bacteria (other)· 1 Organism:C. elegans· 1 Approach:deep learning· 1 Organism:human· 1 Assay:protein structure· 1

Frequent Co-Authors

Roman A. MelnykiD· 2 shared Abigail Shahar GancziD· 1 shared Andrew J. BorstiD· 1 shared Brendan D Snarr· 1 shared Brian CoventryiD· 1 shared Caroline E. Perkins· 1 shared Casey M. TheriotiD· 1 shared Connor WeidleiD· 1 shared

Activity by Year

2015-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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Curved chords = mutual co-authorship

2 shared papersMicrobiology

Roman A. MelnykORCID

Mutual co-authors in cluster

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3 papers

Recent·Most cited

Bile acid dependent attenuation of toxin mediated disease is independent of colonization resistance against C. difficile

Samantha C. Kisthardt, Caroline E. Perkins, Abigail Shahar Gancz, Noah S. Lyons, Stéphanie Thomas, E. Vincent, Guozhi Zhang, John Tam, Roman A. Melnyk, Elizabeth C. Rose, Erin Shammel Baker, Casey M. Theriot

This study shows that secondary bile acid metabolism, not nutrient competition, reduces C. difficile toxin-mediated disease in vivo, independent of colonization resistance.

bioRxiv · Mar 12, 2026 · PreprintMicrobiology

De novo design of potent inhibitors of Clostridioides difficile toxin B

Robert J. Ragotte, Huazhu Liang, John Tam, Sean Miletic, Jacob M. Berman, Roger Palou, Connor Weidle, Zhijie Li, Matthias Glögl, Greg L. Beilhartz, Kenneth D. Carr, Andrew J. Borst, Brian Coventry, Xinru Wang, John L. Rubinstein, , David A. Baker

This paper designs potent small protein inhibitors of C. difficile toxin B using deep learning and Rosetta, showing high affinity and stability for potential clinical use.

Proceedings of the National Academy of Sciences · Sep 22, 2025Peer-reviewedMicrobiology

Exopolysaccharide biosynthetic glycoside hydrolases can be utilized to disrupt and prevent Pseudomonas aeruginosa biofilms

Perrin Baker, Preston J HIll, Brendan D Snarr, Noor Alnabelseya, Mathew J Pestrak, Mark J Lee, Laura K Jennings, John Tam, Roman Melnyk, Matthew R Parsek, Donald C Sheppard, Daniel J Wozniak, P. Lynne Howell

This study demonstrates that glycoside hydrolases PelAh and PslGh can disrupt and prevent Pseudomonas aeruginosa biofilms by degrading exopolysaccharides, enhancing antibiotic efficacy, and improving immune response.

bioRxiv · Nov 26, 2015Microbiology