Casey M. Theriot

Microbially conjugated bile acids differentially affect the lifecycle and pathogenesis of Clostridioides difficile

The paper synthesizes and tests microbially conjugated bile acids (MCBAs) to determine their effects on Clostridioides difficile lifecycle stages, revealing amino acid-dependent inhibitory or promoting effects.

Bile acid dependent attenuation of toxin mediated disease is independent of colonization resistance against C. difficile

This study shows that secondary bile acid metabolism, not nutrient competition, reduces C. difficile toxin-mediated disease in vivo, independent of colonization resistance.

Unlocking the Bile Acid Universe: Advanced Workflows and a Multidimensional Library of 280 Unique Species

The paper develops a multidimensional bile acid reference library and evaluates workflows for accurate bile acid measurement in biological samples.

Assessing contamination in DNA extraction kits commonly used for microbiome research

The paper evaluates contamination in commonly used DNA extraction kits for microbiome research and provides guidance for low biomass measurements.

Clostridioides difficile Toxins Alter Host Metabolic Pathway and Bile Acid Homeostasis Gene Expression In Colonic Epithelium

This study shows that C. difficile toxins alter host gene expression related to metabolic pathways and bile acid homeostasis in colonic epithelial cells.

Distilling Mechanistic Models From Multi-Omics Data

The paper presents a four-step approach to distill mechanistic models from multi-omics data, applied to murine Clostridioides difficile infection data to identify the Stickland reactor's role in toxin production.

Offline Two-dimensional Liquid Chromatography-Mass Spectrometry for Deep Annotation of the Fecal Metabolome

This paper introduces an offline two-dimensional liquid chromatography-mass spectrometry approach to improve metabolome annotation in fecal samples, identifying more compounds than traditional methods.

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution

This paper introduces a high-throughput amplicon sequencing method for full-length 16S rRNA gene analysis with single-nucleotide resolution and near-zero error rate.