High-throughput single-molecule quantification of individual base stacking energies in nucleic acids
J. A. Punnoose, K. J. Thomas, A. R. Chandrasekaran, J. Vilcapoma, A. Hayden, K. Kilpatrick, S. Vangaveti, A. Chen, T. Banco, K. Halvorsen
This study measures individual base stacking energies in DNA and RNA using a custom Centrifuge Force Microscope, finding stronger stacking between purines and weaker between pyrimidines, with implications for DNA nanostructures, enzymatic ligation, and MD simulations.
bioRxiv · May 25, 2022Biophysics