A. Chen

Depletion of CX3CR1+ macrophages results in disrupted functionality and immune surveillance within epididymis and testis

This study shows that CX3CR1+ macrophages are essential for maintaining immune balance and epithelial integrity in the epididymis and testis, with their depletion leading to immune dysfunction and fertility issues.

Identification of targetable vulnerabilities of PLK1-overexpressing cancers by synthetic dosage lethality

The paper identifies synthetic dosage lethality targets for PLK1-overexpressing cancers using genome-wide screens and validates key hits to show their therapeutic potential.

Spatiotemporal transcriptomic profiling reveals the dynamic immunological landscape of alveolar echinococcosis

This study uses spatiotemporal transcriptomics to analyze immune dynamics in E. multilocularis infected mouse liver, identifying key immune cell roles and shifts in immune strategy during infection.

Eco-evolutionary Guided Pathomic Analysis to Predict DCIS Upstaging

This study uses ecological analysis of hypoxia and acidosis biomarkers to improve prediction of DCIS upstaging by identifying habitat-based metabolic phenotypes and spatial niches.

A Coronaviral Pore-Replicase Complex Drives RNA Synthesis in Double Membrane Vesicles

This study identifies nsp4 as the key organizer of coronavirus DMV pores and shows it regulates mRNA exit with implications for antiviral strategies.

An Infusible Extracellular Matrix Biomaterial Improves Survival in a Model of Severe Systemic Inflammation

This study shows that an infusible extracellular matrix biomaterial improves survival in a mouse model of severe systemic inflammation by reducing inflammation and organ failure.

Biomarker Identification by Proteomic Analysis of Vitreous Humor and Plasma in Diabetic Retinopathy

This study identifies protein biomarkers in vitreous humor and plasma that predict diabetic retinopathy progression, with significant changes observed in vitreous samples of PDR patients.

Insulin Resistance Increases TNBC Aggressiveness and Brain Metastasis via Adipocyte-derived Exosomes

This study shows insulin-resistant adipocyte exosomes promote TNBC aggressiveness and brain metastasis via miRNA pathways.

Massively parallel interrogation of human functional variants modulating cancer immunosurveillance

The paper identifies functional mutations affecting PD-L1 and HLA-I expression using a large-scale CRISPR screen, revealing clinically relevant variants that influence immunotherapy response.

Circulating tumor cells shed shearosome extracellular vesicles in capillary bifurcations that activate endothelial and immune cells

This study shows circulating tumor cells shed shearosome extracellular vesicles in capillary bifurcations, which activate endothelial and immune cells and contribute to pre-metastatic niche formation.

A Quantitative Metric of Confidence For Segmentation of Nuclei in Large Spatially Variable Image Volumes

The paper introduces a confidence metric for nuclei segmentation in large spatially variable image volumes, showing it correlates with segmentation quality and F1 scores.

Proteomic profile analysis of plasma and aqueous humor from glaucoma and non-glaucomatous patients

This study compares proteomic profiles of aqueous humor and plasma between glaucoma and non-glaucomatous patients, identifying differentially abundant proteins linked to vascular integrity, inflammation, and neurodegeneration.

Development of BODIPY-based fluorescent probes for highly selective amino acid identification

This paper develops BODIPY-based fluorescent probes for selective amino acid identification through optimized spectral and photophysical properties.

Mechanisms of genomic instability dictate cytosolic DNA composition and dendritic cell mediated anti-tumor immunity

The paper shows that genomic instability in MSI CRCs generates cytosolic DNA that enhances STING activation and anti-tumor immunity via dendritic cells, suggesting improved DNA-based therapies for CIN CRCs.

Biophysics of microRNA-34a targeting and its influence on down-regulation

This study investigates how miR-34a interacts with its mRNA targets and how AGO2 modulates their binding affinity and structure, revealing structural determinants of repression efficacy.

Surface Patterned Omniphobic Tiles (SPOTs): a versatile platform for scalable liquid handling

The paper introduces SPOTs, a low-cost, scalable platform for liquid handling that enables high-throughput experiments with precision and compatibility with existing lab equipment.

Mutation of two intronic nucleotide alters RNA structure and dynamics, inhibiting MBNL1 and RBFOX1 regulated splicing of the Insulin Receptor

The paper shows that mutating two intronic nucleotides alters RNA structure and dynamics, disrupting MBNL1 and RBFOX1 regulation of INSR exon 11 splicing.

Cytotoxic chemotherapy potentiates the immune response and efficacy of combination CXCR4/PD-1 inhibition in models of pancreatic ductal adenocarcinoma

This study shows that combining gemcitabine with CXCR4 inhibitors and anti-PD1 therapy improves immune response and survival in pancreatic cancer models.

Scalable Genotyping of Microbial Colonies

This paper develops a scalable protocol for microbial isolate genotyping using colony PCR and a four-barcode indexing scheme, achieving 90% identification accuracy on 93 environmental samples.

hURAT1 Transgenic Mouse Model for Evaluating Targeted Urate-Lowering Agents

This paper develops a human URAT1 transgenic mouse model to evaluate urate-lowering agents and study URAT1-related pathogenesis in gout.

High-sensitivity whole-genome recovery of single viral species in environmental samples

This paper develops a method to recover complete viral genomes from environmental samples with high sensitivity, achieving 100% recovery of a spiked-in virus and 99.4% for a larger virus.

Global Sensitivity Analysis of the Onset of Nasal Passage Infection by SARS-CoV-2 With Respect to Heterogeneity in Host Physiology and Host Cell-Virus Kinetic Interactions

This study uses global sensitivity analysis to identify key factors driving variability in SARS-CoV-2 nasal infection outcomes, focusing on host physiology and cell-virus kinetics.

Predicting Small Molecule Ligand/RNA Binding Pocket Binding Modes Using Metadynamics

This paper uses metadynamics to predict small molecule-RNA binding modes, showing agreement with experimental structures and providing binding pathways for RNA aptamers.

Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoids

This paper develops a cerebral organoid model of POLG disease that recapitulates its molecular and pathological features, including mtDNA depletion and neuronal loss, and shows metformin can alleviate some abnormalities.

Evolving Membrane-Associated Accessory Protein Variants for Improved Adeno-Associated Virus Production

This study evolves MAAP variants to enhance AAV production, finding that selected variants improve vector genome packaging in multiple AAV serotypes.

Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis

This study shows fungal small RNAs are secreted via extracellular vesicles and enter plant cells through clathrin-mediated endocytosis, using BcPLS1 as an EV biomarker.

Taphonomic damage obfuscates interpretation of the retroarticular region of the Asteriornis mandible

The paper reinterprets the retroarticular region of Asteriornis mandible, showing taphonomic damage obscured its morphology, and updates phylogenetic analysis to support its placement as a total-group galloanseran.

Defining the mechanisms of action and resistance to the anti-PD-1+LAG-3 and anti-PD-1+CTLA-4 combinations in melanoma flank and brain models

This study investigates the mechanisms of action and resistance of anti-PD-1+LAG-3 and anti-PD-1+CTLA-4 combinations in melanoma models, finding differential effects on CD4+ T cell polarization and CD8+ T cell function.

Recruitment of the SNX17-Retriever recycling pathway regulates synaptic function and plasticity.

This study shows that the SNX17-Retriever pathway regulates synaptic function and plasticity by maintaining excitatory synapses and enabling structural changes during long-term potentiation.

Facultative symbiont virulence determines horizontal transmission rate without host strain specificity

This study investigates the evolutionary dynamics of a facultative symbiosis between Dictyostelium discoideum and Paraburkholderia bonniea, finding that host-symbiont associations result from priority effects rather than coevolution.